뽀닥사의 실험노트

Plant DNA minipreparation : Plant genomic DNA mini-prep for PCR reaction (=Preparation of total Arabidopsis DNA) 1. 시약 Lysis buffer (100ml)50mM Tris (2M Tris (pH 7.6) , 2.5ml)100mM NaCl (5M NaCl , 2ml)50mM EDTA (0.5M EDTA , 10ml)83ml ddH2O첨가Autoclave 121도, 15분간0.5% SDS (20% SDS , 2.5ml)10mM [beta]-mercaptoethanol(BME) (14.26M BME, 70.1[mu]l) TEN buffer(100ml)10mM Tris (2M Tris(pH 8.0), 0.5ml)1m..

미세조류 Chlamydomonas rainhardtii 의 DNA 추출하는 방법이다. 시약SDS-EB buffer(2%SDS, 400mM NaCl, 50mM EDTA, 100mM Tris-HCl pH8.0)PCI(25:24:1, phenol:chloroform:isoamylalcohol), pH8.0 (주)바이오세상100% EtOH, 70% EtOHTE bufferRNaseA(20mg/ml, Invitrogen, 12091021) 1. 배양한 cell 중, 1x10^7 cells ~ 2 x 10^7 cells 사용 2. Resuspend cells in 200ul of H2O, add 400ul of SDS-EB buffer, and vortex to mix.200ul H2O 넣은 후 400ul SDS-..